Comparing E342 - Ammonium phosphate vs E429 - peptone
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Popular questions
What is the formula for ammonium phosphate?
For E342, the ammonium phosphates used are monoammonium phosphate (NH4H2PO4) and diammonium phosphate ((NH4)2HPO4); the fully neutral (NH4)3PO4 is unstable and not typically used in foods.
Is monoammonium phosphate toxic?
It has low toxicity and is permitted as a food additive; within the group ADI for phosphates (e.g., EFSA: 40 mg phosphorus/kg body weight/day), normal food uses are considered safe. Very high intakes can disturb mineral balance, and people with kidney disease should manage phosphate intake.
Is ammonium phosphate toxic?
At permitted food levels, ammonium phosphates (E342) are considered safe, with a group ADI for phosphates set by EFSA at 40 mg phosphorus/kg body weight/day. Excessive intake may affect mineral balance and is a concern for those with impaired kidney function.
Is ammonium phosphate soluble in water?
Yes—both monoammonium and diammonium phosphate are readily soluble in water.
What is a peptone?
A peptone is a water‑soluble mix of short peptides, amino acids, and nutrients made by partial hydrolysis of proteins (e.g., casein, meat, or soy); it’s used as a nutrient source in culture media (tryptone is a casein peptone).
What is the purpose of peptone in the media?
It supplies readily assimilable nitrogen (peptides and amino acids), vitamins, and minerals to support microbial growth, including fastidious organisms.
How to make peptone water?
Dissolve 10 g peptone and 5 g sodium chloride in 1 L purified water, adjust pH to about 7.2 at 25°C, dispense, and sterilize by autoclaving at 121°C for 15 minutes.
What is buffered peptone water?
A phosphate‑buffered version of peptone water (per liter: peptone 10 g, NaCl 5 g, Na2HPO4 3.5 g, KH2PO4 1.5 g; pH ~7.2) used as a non‑selective pre‑enrichment medium to resuscitate stressed bacteria, especially Salmonella, from foods.
What is peptone in microbiology?
In microbiology, peptone refers to protein hydrolysates (from casein, meat, soy, etc.) rich in peptides and amino acids that serve as the main nitrogen source in culture media; different peptones (e.g., tryptone) can influence growth and test outcomes.