Comparing E160D - Lycopene vs E124 - Ponceau 4r
Overview
Synonyms
Products
Found in 206 products
Found in 14 products
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Search volume over time
Interest over time for 2 keywords in U.S. during the last 10 years.
Interest over time for 6 keywords in U.S. during the last 10 years.
Popular questions
What is lycopene good for?
As a food additive (E160d), it provides a natural red color to foods; in the diet, it's an antioxidant carotenoid that’s been studied for heart and prostate health, though evidence for disease prevention is mixed.
How much lycopene per day for prostate health?
There’s no established medical dose; clinical studies often use about 10–30 mg/day from tomato products or supplements, but benefits are not confirmed—discuss supplementation with a healthcare professional.
How much lycopene per day?
There’s no RDA, but safety authorities set an acceptable daily intake of 0–0.5 mg/kg body weight/day (about up to 35 mg/day for a 70 kg adult); typical diets provide only a few milligrams per day.
What does lycopene do?
In foods, E160d colors products red and helps standardize appearance; in the body it acts as an antioxidant carotenoid with no vitamin A activity.
What foods have lycopene?
Naturally rich sources include tomatoes and tomato products (paste, sauce, ketchup), watermelon, pink grapefruit, guava, papaya, red carrots, and gac; as an additive, it appears on labels as lycopene or E160d in items like beverages, confectionery, sauces, and dairy desserts.
How does ponceau stain work?
In labs, “Ponceau stain” usually means Ponceau S (not the food color E124 Ponceau 4R); it’s an anionic azo dye that reversibly binds proteins on nitrocellulose or PVDF via electrostatic and hydrophobic interactions, giving red bands. As a similar sulfonated azo dye, E124 would color proteins non‑specifically by the same principle but isn’t the standard reagent.
How to image ponceau stained membrane?
Rinse to lower background, then image the wet membrane under white light with a flatbed scanner, gel imager, or phone camera—no special filters needed. After imaging, destain with water or TBST before immunodetection.
How to make ponceau s solution?
Ponceau S (different from E124) is typically prepared as 0.1% w/v dye in 5% v/v acetic acid in water; mix to dissolve and filter. Store at room temperature; optionally include ~0.1% SDS to speed staining.
How to make ponceau stain?
For protein blots, use Ponceau S: 0.1% w/v in 5% v/v acetic acid (water), filter, and use to briefly stain the membrane. E124 Ponceau 4R is a food colorant and isn’t standard for this application.
How to remove ponceau stain?
Wash the membrane in water or TBST until the red color disappears (typically 1–5 minutes); the staining is fully reversible and compatible with later antibody probing. If background persists, a brief rinse in dilute base (e.g., ~0.1 M NaOH) can clear it quickly.