Comparing E160C - Paprika extract vs E124 - Ponceau 4r
Overview
Synonyms
Products
Found in 8,402 products
Found in 14 products
Search rank & volume
Awareness score
Search volume over time
Interest over time for 9 keywords in U.S. during the last 10 years.
Interest over time for 6 keywords in U.S. during the last 10 years.
Popular questions
What color is paprika?
Paprika extract (E160c) gives orange‑red to deep red hues, appearing yellow‑orange at low use levels and deeper red at higher levels.
Is paprika just for color?
As an additive, paprika extract is used primarily as a coloring; at typical doses it contributes little flavor, though it may add a mild peppery note.
How is paprika extract made?
It’s produced by solvent-extracting ground paprika peppers (Capsicum annuum) with food‑grade solvents like hexane or ethanol to concentrate carotenoids (capsanthin, capsorubin), then removing solvent and standardizing in oil; water‑dispersible forms are made by emulsification or saponification.
What does the color paprika look like?
A warm, natural red—ranging from yellow‑orange to deep red depending on concentration and the product.
What is paprika extract color?
Reddish‑orange to deep red, due to the carotenoids capsanthin and capsorubin.
How does ponceau stain work?
In labs, “Ponceau stain” usually means Ponceau S (not the food color E124 Ponceau 4R); it’s an anionic azo dye that reversibly binds proteins on nitrocellulose or PVDF via electrostatic and hydrophobic interactions, giving red bands. As a similar sulfonated azo dye, E124 would color proteins non‑specifically by the same principle but isn’t the standard reagent.
How to image ponceau stained membrane?
Rinse to lower background, then image the wet membrane under white light with a flatbed scanner, gel imager, or phone camera—no special filters needed. After imaging, destain with water or TBST before immunodetection.
How to make ponceau s solution?
Ponceau S (different from E124) is typically prepared as 0.1% w/v dye in 5% v/v acetic acid in water; mix to dissolve and filter. Store at room temperature; optionally include ~0.1% SDS to speed staining.
How to make ponceau stain?
For protein blots, use Ponceau S: 0.1% w/v in 5% v/v acetic acid (water), filter, and use to briefly stain the membrane. E124 Ponceau 4R is a food colorant and isn’t standard for this application.
How to remove ponceau stain?
Wash the membrane in water or TBST until the red color disappears (typically 1–5 minutes); the staining is fully reversible and compatible with later antibody probing. If background persists, a brief rinse in dilute base (e.g., ~0.1 M NaOH) can clear it quickly.