Comparing E124 - Ponceau 4r vs E163 - Anthocyanins
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Found in 14 products
Found in 260 products
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Interest over time for 6 keywords in U.S. during the last 10 years.
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Popular questions
How does ponceau stain work?
In labs, “Ponceau stain” usually means Ponceau S (not the food color E124 Ponceau 4R); it’s an anionic azo dye that reversibly binds proteins on nitrocellulose or PVDF via electrostatic and hydrophobic interactions, giving red bands. As a similar sulfonated azo dye, E124 would color proteins non‑specifically by the same principle but isn’t the standard reagent.
How to image ponceau stained membrane?
Rinse to lower background, then image the wet membrane under white light with a flatbed scanner, gel imager, or phone camera—no special filters needed. After imaging, destain with water or TBST before immunodetection.
How to make ponceau s solution?
Ponceau S (different from E124) is typically prepared as 0.1% w/v dye in 5% v/v acetic acid in water; mix to dissolve and filter. Store at room temperature; optionally include ~0.1% SDS to speed staining.
How to make ponceau stain?
For protein blots, use Ponceau S: 0.1% w/v in 5% v/v acetic acid (water), filter, and use to briefly stain the membrane. E124 Ponceau 4R is a food colorant and isn’t standard for this application.
How to remove ponceau stain?
Wash the membrane in water or TBST until the red color disappears (typically 1–5 minutes); the staining is fully reversible and compatible with later antibody probing. If background persists, a brief rinse in dilute base (e.g., ~0.1 M NaOH) can clear it quickly.
Is anthocyanin always present in leaves?
No—anthocyanins vary by species and conditions; many green leaves have little to none until they’re young, senescing (autumn), or under stress such as high light, cold, or nutrient limitation.
How much anthocyanin per day?
There’s no recommended daily intake or established ADI; typical diets supply roughly a few tens to a few hundred milligrams per day from fruits and vegetables, and intake at normal food levels is considered safe.
What colors come from anthocyanins?
They give red, pink, purple, and blue hues, shifting with pH—more red in acidic conditions and more blue/purple as pH rises.
How to extract anthocyanin from plants?
Crush colored plant material and soak it in acidified water or food-grade ethanol (e.g., 50–70% ethanol or water with a little lemon juice), then filter; keep the extract cool, protected from light, and away from high pH to limit degradation.
What are anthocyanins good for?
As E163, they’re used to color foods and drinks in red-to-blue shades; while they show antioxidant activity in vitro, human health benefits remain limited and inconclusive.