Comparing E124 - Ponceau 4r vs E127 - Erythrosine
Overview
Synonyms
Products
Found in 14 products
Found in 5,789 products
Search rank & volume
Awareness score
Search volume over time
Interest over time for 6 keywords in U.S. during the last 10 years.
Interest over time for 9 keywords in U.S. during the last 10 years.
Popular questions
How does ponceau stain work?
In labs, “Ponceau stain” usually means Ponceau S (not the food color E124 Ponceau 4R); it’s an anionic azo dye that reversibly binds proteins on nitrocellulose or PVDF via electrostatic and hydrophobic interactions, giving red bands. As a similar sulfonated azo dye, E124 would color proteins non‑specifically by the same principle but isn’t the standard reagent.
How to image ponceau stained membrane?
Rinse to lower background, then image the wet membrane under white light with a flatbed scanner, gel imager, or phone camera—no special filters needed. After imaging, destain with water or TBST before immunodetection.
How to make ponceau s solution?
Ponceau S (different from E124) is typically prepared as 0.1% w/v dye in 5% v/v acetic acid in water; mix to dissolve and filter. Store at room temperature; optionally include ~0.1% SDS to speed staining.
How to make ponceau stain?
For protein blots, use Ponceau S: 0.1% w/v in 5% v/v acetic acid (water), filter, and use to briefly stain the membrane. E124 Ponceau 4R is a food colorant and isn’t standard for this application.
How to remove ponceau stain?
Wash the membrane in water or TBST until the red color disappears (typically 1–5 minutes); the staining is fully reversible and compatible with later antibody probing. If background persists, a brief rinse in dilute base (e.g., ~0.1 M NaOH) can clear it quickly.
What foods have red dye 3?
Common examples are maraschino/cocktail and candied/glacé cherries; some candies, pink frostings/icing gels, cake decorations/sprinkles, and certain snack cakes or bakery items. In the EU it’s mostly limited to cocktail/candied cherries, while in the U.S. it appears in a wider range of confections and decorations.
What has red dye 3 in it?
Products that often use it include maraschino/cocktail and candied/glacé cherries, some candies, pink frostings/icing gels, cake decorations/sprinkles, and certain snack cakes or pastries. EU rules largely restrict it to cocktail/candied cherries; U.S. uses are broader in confections and decorations.
What food has red dye 3?
Maraschino/cocktail and candied/glacé cherries are the most common; it’s also found in some candies, pink frostings/icing gels, cake decorations/sprinkles, and occasional snack cakes or bakery items. Availability varies by region, with the EU mostly limiting it to preserved cherries.
Will there be a red dead redemption 3?
That’s a video game question and not related to the food color E127 (Red 3); if you meant foods with Red 3, typical ones are maraschino/candied cherries, some candies, and pink frostings/cake-decorating gels.
What foods contain red dye 3?
Frequently: maraschino/cocktail and candied/glacé cherries; some candies; pink frostings/icing gels; cake decorations/sprinkles; and certain snack cakes or bakery items. In the EU it is largely restricted to preserved cherries.